食環署也非從未引用現有法律對寵物友善餐廳執法：2025年2月，大埔大元商場一家咖啡廳因兩度被抓獲允許狗隻進入，被法庭定罪罰款後，再被食環署吊銷牌照七天，引起輿論和狗主討論。
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This reaction would contain only low concentrations of the dNTPs and relied upon brief incubation times, so that DNA synthesis would stall at random positions along the template and yield a population of DNA fragments with varying lengths. These unfinished DNA fragments were then purified and used as templates in four “minus” and four “plus” reactions.