Besides the Test PLA, the 386 has another PLA called the Entry PLA that maps opcodes to microcode entry points. One of its input bits is a "protected mode" flag. Many instructions have both a real-mode and a protected-mode entry point -- for instance, MOV ES, reg maps to address 009 (a single microcode line) in real mode, but to 580 (which initiates a full descriptor load with protection tests) in protected mode. The trick that makes V86 work is to define this flag as:
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But of course, like any immutable system, there are mutable parts (otherwise, we couldn’t create any configuration files). OSTree handles this with “overlays” (actually, we use OverlayFS) that allow a read-write filesystem to be layered on top of the immutable system. For example, the /etc and /var directories are writable, while the rest of the system is read-only.